Regulatory

Part:BBa_K2680528

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-13)


pTlpAr

pTlpAr is the reverse complement of pTlpA, a promoter repressed by heat-sensitive protein TlpA. pTlpAr transcribes 200-fold weaker than pTlpA.1 Flanked by prefix sticky end A and suffix sticky end B.

References

1Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None